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BioSim™ Secukinumab (Human) ELISA Kit

A Sandwich ELISA kit for the Quantitative measurement of free Secukinumab in serum or plasma samples

WARNING: This product can expose you to chemicals including TMB, which is known to the State of California to cause cancer, For more information go to
Catalog #: E5026

Product Details

Cat # +Size E5026-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications The Sandwich ELISA kit is used for in vitro Quantitative determination of free Secukinumab in human serum and plasma samples
Features & Benefits ● Sensitivity: 6.25 ng/ml
● For in vitro Quantitative determination of free Secukinumab in human serum and plasma samples
● Cross Reactivity: Except for Secukinumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
● Detection Range: 6.25 - 100 ng/ml
Kit Components ● Microtiter Plate
● Secukinumab Standard S1 (1000 ng/ml)
● Secukinumab Standard S2 (500 ng/ml)
● Secukinumab Standard S3 (250 ng/ml)
● Secukinumab Standard S4 (125 ng/ml)
● Secukinumab Standard S5 (62.5 ng/ml)
● Secukinumab Standard S6 (0 ng/ml)
● Secukinumab Standard S7 (High Control)
● Secukinumab Standard S8 (Low Control)
● Assay Buffer
● HRP-conjugate Probe
● TMB substrate (Avoid light)
● Stop Solution Wash buffer (20X) Plate sealers
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Secukinumab is a human IgG1 monoclonal antibody that targets IL-17α and prevents its association with the receptor IL-17R. This results in decreased release of proinflammatory cytokines and chemokines and downregulation of inflammation. IL-17 represents a family of cytokines (IL-17 A-F) released by T-helper-17 (Th17) cells, mast cells, neutrophils, and dendritic cells and promotes inflammation. Studies show that IL-17 is associated with multiple autoimmune diseases such as rheumatoid arthritis, spondyloarthritis, psoriasis, Crohn's disease, and multiple sclerosis. The original monoclonal antibody received approval from the FDA to treat plaque psoriasis, psoriatic arthritis, and ankylosing spondylitis. BioSim™ Secukinumab ELISA kit measures free Secukinumab in human serum or plasma with high sensitivity and reproducibility. The kit is based on the Sandwich ELISA principle. Standards and samples (serum or plasma) are added to the microtiter plate coated with the reactant for Secukinumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Secukinumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Secukinumab in the sample or standard. The sample results can be determined directly using the standard curve.

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