Rituximab ELISA Kit (ab237640)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Rituximab ELISA Kit
See all Rituximab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 30% Inter-assay Sample n Mean SD CV% Overall < 30% -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
3 ng/ml -
Range
3 ng/ml - 300 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Note: Rituximab ELISA kit ab237640 is intended for Research Use Only, not for use in diagnostic procedures.
This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Rituximab in the sample or standard. Results of samples can be determined directly using the standard curve.
This assay measures only free Rituximab.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4371 BioSim™ Rituximab (Human) ELISA Kit. E4371-100 is the same size as the 96 test size of ab237640.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 96 tests 96 tests Assay Buffer 1 x 50ml 1 x 50ml 1 x 50ml HRP-conjugate Probe 1 x 12ml 1 x 12ml 1 x 12ml Micro ELISA Plate 1 unit 1 unit 1 unit Plate sealers 2 units 2 units 2 units Rituximab Standard S1 1 x 1ml 1 x 1ml 1 x 1ml Rituximab Standard S2 1 x 1ml 1 x 1ml 1 x 1ml Rituximab Standard S3 1 x 1ml 1 x 1ml 1 x 1ml Rituximab Standard S4 1 x 1ml 1 x 1ml 1 x 1ml Rituximab Standard S5 1 x 1ml 1 x 1ml 1 x 1ml Rituximab Standard S6 1 x 1ml 1 x 1ml 1 x 1ml Rituximab Standard S7 1 x 1ml 1 x 1ml 1 x 1ml Stop Solution 1 x 12ml 1 x 12ml 1 x 12ml TMB substrate 1 x 12ml 1 x 12ml 1 x 12ml Wash buffer (20X) 1 x 50ml 1 x 50ml 1 x 50ml -
Research areas
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Relevance
Rituximab is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of Rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells and is used in the treatment of leukemia s and lymphomas, some autoimmune disorders, and organ transplant. -
Cellular localization
Membrane; Multipass membrane protein. -
Alternative names
- B lymphocyte antigen CD20
- B lymphocyte surface antigen B1
- Bp35
see all -
Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237640 has not yet been referenced specifically in any publications.