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BioSim™ Ramucirumab (Cyramza®)(Human) ELISA Kit

This ELISA kit is used for in vitro quantitative determination of Ramucirumab
Catalog #: E4698
$1,225.00

Product Details

Cat # +Size E4698-100
Size 96 assays
Detection Method colorimetric
Species Reactivity Human
Applications • Sensitivity: 20 ng/ml
• Detection Range: 20 - 600 ng/ml
• Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
• Cross Reactivity: Except for Ramucirumab, there is no cross reaction with other therapeutic antib
Features & Benefits • Sensitivity: 20 ng/ml,Detection Range: 20 - 600 ng/ml
• Cross Reactivity: Except for Ramucirumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.
• This ELISA kit is used for in vitro quantitative determination of Ramucirumab
• Recovery rate: 85 – 115% with normal human serum samples with known concentrations
• Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
Kit Components • Ramucirumab Standards
• Plate sealers
• Wash buffer (20X)
• Stop Solution
• Micro ELISA Plate
• TMB substrate (Avoid light)
• HRP-conjugate Probe
• Assay Buffer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Ramucirumab is a human monoclonal antibody (IgG1) against vascular endothelial growth factor receptor 2 (VEGFR2), a type II trans-membrane tyrosine kinase receptor expressed on endothelial cells. By binding to VEGFR2, Ramucirumab prevents binding of its ligands (VEGF-A, VEGF-C, and VEGF-D), thereby preventing VEGF-stimulated receptor phosphorylation and downstream ligand-induced proliferation, permeability, and migration of human endothelial cells. BioSim™ Vedolizumab ELISA kit is a Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Ramucirumab (Cyramza®). After incubation, the wells are washed. A horse radish peroxidase (HRP) conjugated probe is added and binds to Ramucirumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Ramucirumab in the sample or standard.


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What is the exact "reactant" coated on the plate and could you please provide detailed information on "HRP-conjugate Probe". Is it an antibody against Ramucirumab?
The assay is a solid-phase enzyme immunoassay based on the sandwich principle, where the antigen for ramucirumab is coated on the ELISA plate. Free Ramucirumab from the sample (serum or plasma) binds to the antigen on the surface of the wells, which is then detected by the HRP conjugated secondary probe.