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BioSim™ Pembrolizumab (Human) ELISA Kit

A sandwich ELISA for the quantitative analysis of free pembrolizumab in human serum and plasma samples

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4383
$1,210.00

Product Details

Cat # +Size E4383-100
Size 100 assays
Kit Summary BioSim™ Pembrolizumab ELISA Kit is a sandwich ELISA for the quantitative analysis of free pembrolizumab in human serum and plasma samples.
Detection Method Absorbance at 450 nm
Species Reactivity Human
Applications For the in vitro quantitative determination of free pembrolizumab in serum and plasma samples.
Features & Benefits • Sensitivity: 30 ng/ml
• Cross Reactivity: There is no cross reactivity with native serum immunoglobulins.
• The kit offers easy, fast and sensitive method to quantitate free pembrolizumab in serum and plasma samples.
• Recovery rate: < 100 ± 30%
• Assay Precision: Intra-Assay and inter-assay CVs < 30%
• Detection Range: 30 - 1000 ng/ml
Kit Components • Pembrolizumab Ab coated ELISA Plate
• Pembrolizumab Standards (S1 – S7)
• Assay Buffer
• HRP-Conjugate
• TMB Substrate
• Stop Solution
• Wash Buffer (10X)
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Pembrolizumab is a highly selective IgG4-kappa humanized monoclonal antibody against the programmed cell death protein 1 (PD-1) receptor. It binds to the PD-1 receptor with high affinity and inhibits the interaction of PD-1 receptor with its known ligands PD-L1 and PD-L2. In normal circumstances, the binding of PD-L1 and PD-L2 to PD-1 receptor inhibits the TCR mediated T cell proliferation and cytokine production. But the binding of pembrolizumab to PD-1 receptor, prevents the inhibitory pathway thereby enhancing tumor immunosurveillance and anti-tumor immune responses. BioSim™ Pembrolizumab ELISA Kit is a sandwich ELISA for the quantitative analysis of free pembrolizumab in human serum and plasma samples. In this assay, standards and samples (serum or plasma) are incubated in the ELISA plate coated with anti-pembrolizumab antibody. After incubation, the wells are washed and a HRP conjugated antibody is added, which binds to the pembrolizumab captured by the anti-pembrolizumab antibody in the wells. Following incubation, the wells are then washed and the bound enzymatic activity is detected by the addition of TMB substrate. Finally, the reaction is terminated using stop solution. The color developed is directly proportional to the amount of pembrolizumab in the sample or standard. The kit offers easy, fast and sensitive method to quantitate free pembrolizumab in serum and plasma samples.


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