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BioSim™ Evolocumab (Human) ELISA Kit

A Sandwich ELISA kit for the Quantitative measurement of free Evolocumab in serum or plasma samples

WARNING: This product can expose you to chemicals including TMB, which is known to the State of California to cause cancer, For more information go to
Catalog #: E5025

Product Details

Cat # +Size E5025-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications The Sandwich ELISA kit is used for in vitro Quantitative determination of free Evolocumab in human serum and plasma samples
Features & Benefits ● Sensitivity: 6.25 ng/ml
● For in vitro Quantitative determination of free Evolocumab in human serum and plasma samples
● Cross Reactivity: Except for Evolocumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
● Detection Range: 6.25 - 100 ng/ml
Kit Components ● Microtiter Plate
● Evolocumab Standard S1 (1000 ng/ml)
● Evolocumab Standard S2 (500 ng/ml)
● Evolocumab Standard S3 (250 ng/ml)
● Evolocumab Standard S4 (125 ng/ml)
● Evolocumab Standard S5 (62.5 ng/ml)
● Evolocumab Standard S6 (0 ng/ml)
● Evolocumab Standard S7 (High Control)
● Evolocumab Standard S8 (Low Control)
● Assay Buffer
● HRP-conjugate Probe
● TMB substrate (Avoid light)
● Stop Solution
● Wash buffer (20X)
● Plate sealers
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Evolocumab is a human IgG2 monoclonal antibody that targets proprotein convertase subtilisin/kexin type 9 (PCSK9). PCSK9 is a serine protease that binds to LDL receptors on the hepatocyte cell surface and promotes the lysosome-mediated degradation of these receptors. This results in decreased uptake and clearance of LDL-C (bad cholesterol) by the receptors. The monoclonal antibody binds to PCSK9 and prevents interaction with the LDL receptor. This promotes the recycling of the internalized LDL receptor to the hepatocyte cell surface and increased removal of LDL-C from the blood. The original drug received approval from the FDA to treat homozygous and heterozygous familial cholesterolemia. The drug has also been approved to treat patients with high cholesterol, whose LDL-C was not controlled by the statin treatment. BioSim™ Evolocumab ELISA kit measures free Evolocumab in human serum or plasma with high sensitivity and reproducibility. The kit is based on the Sandwich ELISA principle. Standards and samples (serum or plasma) are added to the microtiter plate coated with the reactant for Evolocumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Evolocumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Evolocumab in the sample or standard. The sample results can be determined directly using the standard curve.

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