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BioSim™ Durvalumab (Human) ELISA Kit

A Sandwich ELISA kit for the Quantitative measurement of free Durvalumab in human serum or plasma samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause birth defects or other reproductive harm.  For more information go to
Catalog #: E4925

Product Details

Cat # +Size E4925-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications The Sandwich ELISA kit is used for in vitro Quantitative determination of free Durvalumab in human serum and plasma samples
Features & Benefits • Detection Range: 10 - 300 ng/ml
• Recovery rate: < 100 ± 30% with known concentrations of normal human serum samples
• For in vitro Quantitative determination of free Durvalumab in human serum and plasma samples
• Cross Reactivity: Except for Durvalumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
• Sensitivity: 10 ng/mL
• Assay Precision: Intra-Assay and Inter-Assay CV < 30%
Kit Components • Microtiter Plate
• Durvalumab Standards (S1 – S7)
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Durvalumab is a human IgG1 monoclonal antibody that binds to and inhibits programmed cell death ligand 1 (PD-L1). PD-L1 is an important immune checkpoint molecule expressed on tumor cells and tumor-infiltrating immune cells. PD-L1 binds to its receptors PD-1 and B7.1 located on the surface of activated T cells and antigen-presenting cells and in turn suppresses cytotoxic T-cell activity, the proliferation of T-cells, and production of cytokines. The monoclonal antibody binds to PD-L1 and inhibits its anti-tumor immune response, and thus reduces tumor growth without causing antibody-dependent cellular cytotoxicity. The original antibody is approved to treat locally advanced or metastatic urothelial carcinoma and locally advanced unresectable non-small cell lung cancer (NSCLC). BioSim™ Durvalumab ELISA kit measures free Durvalumab concentration in human serum or plasma with high sensitivity and reproducibility. The kit is based on the Sandwich ELISA principle. Standards and samples (serum or plasma) are added to the microtiter plate coated with the reactant for Durvalumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Durvalumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Durvalumab in the sample or standard. The sample results can be determined directly using the standard curve.

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