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BioSim™ Canakinumab (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of free Canakinumab in human serum or plasma samples
Catalog #: E4867

Product Details

Cat # +Size E4867-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications The Sandwich ELISA kit is used for in vitro quantitative determination of Canakinumab in human serum and plasma samples
Features & Benefits • Sensitivity: 3 ng/mL
• Detection Range: 3-100 ng/ml
• Cross Reactivity: Except for Canakinumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
• Recovery rate: < 100 ± 30% with known concentrations of normal human serum samples
• Assay Precision: Intra-Assay and Inter-Assay CV < 30%
• For in vitro quantitative determination of Canakinumab in human serum and plasma samples
Kit Components • Microtiter Plate
• Canakinumab Standards (S1 – S7)
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Canakinumab is a recombinant human IgG1 kappa monoclonal antibody that binds to and inhibits human IL-1β by preventing the interaction with its receptor and subsequent inflammatory activity. The antibody neither has cross-reactivity with IL-1α nor with the Interleukin 1 receptor, type I (IL1R1). The antibody has been approved to treat cryopyrin-associated periodic syndromes (CAPS). BioSim™ Canakinumab ELISA kit has been developed for specific quantification of Canakinumab concentration in human serum or plasma with high sensitivity and reproducibility. The kit is based on the sandwich ELISA principle. Standards and samples (serum or plasma) are added in the microtiter plate coated with the reactant for Canakinumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Canakinumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the bound enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Canakinumab in the sample or standard. The results of samples can be determined directly using the standard curve.

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