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BioSim™ Bevacizumab (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Bevacizumab in human serum or plasma.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4373

Product Details

Cat # +Size E4373-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of Bevacizumab in human serum and plasma
Features & Benefits • Easy, convenient, sensitive and time-saving method to measure the level of Bevacizumab in human serum and plasma.
• Detection Range: 30 - 1000 ng/ml
• Sensitivity: 30 ng/ml
• Assay Precision: Intra-Assay: CV < 30%; Inter-Assay: CV < 30% (CV (%) = SD/mean X 100)
• Recovery rate: <100±30% with normal human serum samples with known concentrations
• Cross Reactivity: No significant cross-reactivity or interference with other proteins present in native human serum or other therapeutic immunoglobulins.
Kit Components • Micro ELISA Plate
• Bevacizumab Standards (S1 – S7)
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Bevacizumab is a recombinant human IgG1 monoclonal antibody specific for all human vascular endothelial growth factor-A (VEGF-A) isoforms. The humanized anti-VEGF monoclonal antibody, bevacizumab, has been approved by the FDA as a first-line treatment for metastatic colorectal cancer in combination with chemotherapy. The pharmacokinetic properties of bevacizumab in several species have been previously described and are consistent with a typical humanized monoclonal antibody. It was shown in the literature that the surveillance of circulating concentration during maintenance therapy represents a direct and/or indirect factor for some other side effects. Identification of biomarkers for (non-)response and risk factors for adverse drug reactions that might be related to serum concentrations and maintaining the effective concentration of Bevacizumab in order to potentially avoid some side effects with a reliable method might be beneficial. BioVision’s BioSimTM Bevacizumab ELISA kit has been developed for specific quantification of Bevacizumab concentration in human serum or plasma with high sensitivity and reproducibility.

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What is the principle of the ELISA kit? Could you give us some details?
This is a solid phase ELISA based on the double antigen assay principle. The 96 well plate is coated with human VEGF protein and a biotin conjugated human VEGF is added to which the Bevacizumab binds. Following the recommended incubations, wells are washed and HRP conjugated probe is added for colorimetric detection.