BioSim™ Bevacizumab (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Bevacizumab in human serum or plasma.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4373 | abID: ab237642

Product Details

abID ab237642
Cat # +Size E4373-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of Bevacizumab in human serum and plasma
Features & Benefits • Easy, convenient, sensitive and time-saving method to measure the level of Bevacizumab in human serum and plasma.
• Detection Range: 30 - 1000 ng/ml
• Sensitivity: 30 ng/ml
• Assay Precision: Intra-Assay: CV < 30%; Inter-Assay: CV < 30% (CV (%) = SD/mean X 100)
• Recovery rate: <100±30% with normal human serum samples with known concentrations
• Cross Reactivity: No significant cross-reactivity or interference with other proteins present in native human serum or other therapeutic immunoglobulins.
Kit Components • Micro ELISA Plate
• Bevacizumab Standards (S1 – S7)
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Bevacizumab is a recombinant human IgG1 monoclonal antibody specific for all human vascular endothelial growth factor-A (VEGF-A) isoforms. Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab binds VEGF and prevents vascular endothelial growth and endothelial cell proliferation. BioSimTM Bevacizumab (Human) ELISA Kit has been developed for specific quantification of Bevacizumab concentration in human serum or plasma with high sensitivity and reproducibility. The ELISA kit is based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for bevacizumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to bevacizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of bevacizumab in the sample or standard. Results of samples can be determined directly using the standard curve.


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What is the principle of the ELISA kit? Could you give us some details?
This is a solid phase ELISA based on the double antigen assay principle. The 96 well plate is coated with human VEGF protein and a biotin conjugated human VEGF is added to which the Bevacizumab binds. Following the recommended incubations, wells are washed and HRP conjugated probe is added for colorimetric detection.