BioSim™ Avelumab (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Avelumab in human serum or plasma.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4556 | abID: ab237669

Product Details

abID ab237669
Cat # +Size E4556-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of Avelumab in human serum and plasma
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of Avelumab in human serum and plasma
Features & Benefits • Easy, convenient, sensitive and time-saving method to measure the level of Avelumab in human serum and plasma.
• Detection Range: 100 - 3000 ng/ml
• Sensitivity: 30 ng/ml
• Cross Reactivity: No significant cross-reactivity or interference with other proteins present in native human serum or other therapeutic immunoglobulins.
• Assay Precision: Intra-Assay: CV < 30%; Inter-Assay: CV < 30% (CV (%) = SD/mean X 100)
• Recovery rate: <100±30% with normal human serum samples with known concentrations
Kit Components • Micro ELISA Plate
• Avelumab Standards (S1 – S7)
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Avelumab is a fully human anti-PD-L1 IgG1 lambda monoclonal antibody that has a molecular weight of approximately 147 kDa. Avelumab binds PD-L1 and blocks the interaction between PD-L1 and its receptors PD-1 and B7-1. By inhibiting PD-L1 interactions, avelumab is thought to enable the activation of T-cells and the adaptive immune system. By retaining anative Fc-region, avelumab is thought to engage the innate immune system and may induce antibody-dependent cell-mediated cytotoxicity. BioSim™ Avelumab (Human) ELISA Kit has been developed for specific quantification of Avelumab concentration in human serum or plasma with high sensitivity and reproducibility. The ELISA kit is based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for avelumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to avelumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of avelumab in the sample or standard. Results of samples can be determined directly using the standard curve.


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