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BioSim™ anti-Ustekinumab (Human) ELISA Kit

A Sandwich ELISA kit for the qualitative measurement of antibodies to Ustekinumab in human serum or plasma samples
Catalog #: E4872

Product Details

Cat # +Size E4872-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is used for in vitro qualitative determination of antibodies to Ustekinumab in human serum and plasma samples
Features & Benefits • Assay Precision: Intra-Assay and Inter-Assay CV < 30%
• For in vitro qualitative determination of antibodies to Ustekinumab in human serum and plasma samples
• Cross Reactivity: Except for Ustekinumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
Kit Components • Microtiter Plate
• Positive Control
• Negative Control
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ustekinumab is a fully human IgG1 monoclonal antibody that targets the p40 subunit of IL-12 and IL-23, thereby preventing their association with IL-12Rβ1 receptor expressed on NK cells and T-cell surface. As a result, the antibody inhibits IL-12 and IL-23 mediated signaling, activation, and subsequent inflammatory response. The antibody has been approved to treat moderate-to-severe plaque psoriasis, Crohn’s disease, and ulcerative colitis. BioSim™ anti-Ustekinumab ELISA kit has been specifically developed to qualitatively determine antibodies to Ustekinumab in human serum and plasma samples. The ELISA kit is based on the sandwich principle. Controls and samples are incubated in the microtiter plate coated with the drug Ustekinumab. After incubation, the wells are washed and the enzymatic activity is detected by the addition of chromogen-substrate. The enzyme-substrate reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies specific for Ustekinumab present in the samples and controls. The qualitative test results can be evaluated using cut-off value.

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