Product Details
| abID | ab282911 |
|---|---|
| Cat # +Size | E4859-100 |
| Size | 96 assays |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Species Reactivity | Human |
| Applications | This ELISA kit is used for in vitro qualitative determination of antibody against Tocilizumab in serum and plasma |
| Features & Benefits | • Cross Reactivity: Tocilizumab infusion camouflages/masks the presence of antibody to Tocilizumab in serum/plasma samples. Therefore, blood sampling time is critical for detection of anti-drug-antibodies. It is convenient to obtain blood sample just before the infusion or at least 2 weeks after the infusion of Tocilizumab. • This ELISA kit is used for in vitro qualitative determination of antibody against Tocilizumab in serum and plasma |
| Kit Components | • Micro ELISA Plate
• Positive Control • Negative Control • Assay Buffer • Peroxidase Conjugate • TMB substrate (Avoid light) • Stop Solution • Wash buffer (20X) • Plate sealers |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Tocilizumab is a recombinant, humanized, anti-human interleukin 6 (IL-6) receptor monoclonal antibody. The light chain is made up of 214 amino acids. The heavy chain is made up of 448 amino acids. The four polypeptide chains are linked intra- and intermolecularly by disulfide bonds. BioVision’s BioSim™ anti-Tocilizumab ELISA Kit is designed to detect antibody against Tocilizumab with high specificity and sensitivity in serum and plasma samples. Anti-Tocilizumab ELISA is based on the sandwich ELISA principle. Controls and samples are incubated in the microtiter plate coated with Tocilizumab. After incubation, the wells are washed. Then, HRP conjugated probe is added and binds to Tocilizumab antibodies captured by Tocilizumab on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Tocilizumab antibodies in the sample or controls. The results can be evaluated with using cut-off value.
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