Product Details
| abID | ab237668 |
|---|---|
| Cat # +Size | E4399-100 |
| Size | 100 assays |
| Kit Summary | • Detection method- Absorbance (450 nm) • Species reactivity- Human • Application- This ELISA kit is used for quantitative measurement of antibody against Filgrastim in human serum and plasma |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Human |
| Applications | This ELISA kit is used for quantitative measurement of antibody against Filgrastim in human serum and plasma |
| Features & Benefits | • Cross Reactivity: Filgrastim infusion camouflages/masks the presence of antibody to Filgrastim (ATT) in serum/plasma samples. Therefore, blood sampling time is critical for detection of ATT. It is convenient to obtain blood sample just before the infusion of Filgrastim or at least 2 weeks after the infusion of Filgrastim.
• Detection Range: 3.1 - 50 ng/ml • Sensitivity: 3.1 ng/ml • Easy, convenient, sensitive and time-saving method to measure the level of antibody against Filgrastim in human serum and plasma. |
| Kit Components | • Micro ELISA Plate
• Filgrastim Standards (S1) • Filgrastim Standards (S2) • Filgrastim Standards (S3) Filgrastim Standards (S4) • Filgrastim Standards (S5) • Filgrastim Standards (S6) • Filgrastim Standards (S7) • Filgrastim Standards (S8) • Assay Buffer • Peroxidase-Conjugate • TMB substrate (Avoid light) • Stop Solution • Wash buffer (20X) • Confirmation Reagent • Plate sealers |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Filgrastim is a recombinant, non-pegylated human granulocyte colony-stimulating factor (G-CSF) analog. It binds to the G-CSF receptor and stimulates the production of neutrophils in the bone marrow. As a G-CSF analog, it controls the proliferation of committed progenitor cells and influences their maturation into mature neutrophils. BioSimTM anti-Filgrastim (Human) ELISA Kit is designed to detect antibodies against Filgrastim with high specificity and sensitivity in biological matrices. The kit is based on the sandwich principle. Controls and samples are incubated in the microtiter plate coated with the Filgrastim. After incubation, the wells are washed, and the enzymatic activity is detected by the addition of chromogen-substrate. The enzyme-substrate reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies specific for Filgrastim present in the samples and standards. The quantitative test results can be determined using the standard curve.
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