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BioSim™ anti-Filgrastim (Neupogen®) (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of antibody against Filgrastim in human serum or plasma.
Catalog #: E4399

Product Details

Cat # +Size E4399-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of antibody against Filgrastim in human serum and plasma
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of antibody against Filgrastim in human serum and plasma
Features & Benefits • Easy, convenient, sensitive and time-saving method to measure the level of antibody against Filgrastim in human serum and plasma.
• Detection Range: 3.1 - 50 ng/ml
• Sensitivity: 3 ng/ml
• Assay Precision: Intra-Assay: CV < 30%; Inter-Assay: CV < 30% (CV (%) = SD/mean X 100)
• Recovery rate: <100±30% with normal human serum samples with known concentrations
• Cross Reactivity: Filgrastim (Neupogen®) infusion camouflages/masks the presence of antibody to Filgrastim (ATT) in serum/plasma samples. Therefore, blood sampling time is critical for detection of ATT. It is convenient to obtain blood sample just before the infusion of Filgrastim or at least 2 weeks after the infusion of Filgrastim.
Kit Components • Micro ELISA Plate
• Filgrastim Standards (S1 – S8)
• Assay Buffer
• Biotin Conjugate (100X)
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Confirmation Reagent
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Filgrastim (Neupogen®) is a recombinant, non-pegylated human granulocyte colony stimulating factor (G-CSF) analogue. It binds to the G-CSF receptor and stimulates the production of neutrophils in the bone marrow. As a G-CSF analog, it controls proliferation of committed progenitor cells and influences their maturation into mature neutrophils. Filgrastim is used in patients with acute myeloid leukemia receiving induction or consolidation chemotherapy. It is also used in cancer patients receiving bone marrow transplant. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects. BioVision’s BioSimTM anti-Filgrastim ELISA kit is designed to detect antibody against Filgrastim with high specificity and sensitivity in biological matrices.

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