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BioSim™ anti-Canakinumab (Human) ELISA Kit

A Sandwich ELISA kit for the qualitative measurement of antibodies to Canakinumab in human serum or plasma samples
Catalog #: E4868

Product Details

Cat # +Size E4868-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is used for in vitro qualitative determination of antibodies to Canakinumab in human serum and plasma samples
Features & Benefits • For in vitro qualitative determination of antibodies to Canakinumab in human serum and plasma samples
• Assay Precision: Intra-Assay and Inter-Assay CV < 30%
• Cross Reactivity: Except for Canakinumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
Kit Components • Microtiter Plate
• Positive Control
• Negative Control
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Canakinumab is a recombinant human IgG1 kappa monoclonal antibody that binds to and inhibits human IL-1β by preventing the interaction with its receptor and subsequent inflammatory activity. The antibody neither has cross-reactivity with IL-1α nor with the Interleukin 1 receptor, type I (IL1R1). The antibody has been approved to treat cryopyrin-associated periodic syndromes (CAPS). BioSim™ anti-Canakinumab ELISA kit has been developed to detect antibodies to Canakinumab in human serum or plasma. The kit is based on the sandwich ELISA principle wherein controls and samples (serum or plasma) are added in the microtiter plate coated with Canakinumab. After incubation, the wells are washed and the enzymatic activity is detected by the addition of chromogen-substrate. The enzyme-substrate reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies specific for Canakinumab present in the samples and controls. The qualitative test results can be evaluated using cut-off value.

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