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BioSim™ anti-Aflibercept (Eylea®) (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of antibody against Aflibercept in human serum or plasma.
Catalog #: E4855

Product Details

Cat # +Size E4855-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for qualitative determination of antibody against Aflibercept in serum and plasma
Features & Benefits • Cross Reactivity: Aflibercept (Eylea®) infusion camouflages/masks the presence of antibody to Aflibercept in serum/plasma samples. Therefore, blood sampling time is critical for detection of anti-drug-antibodies. It is convenient to obtain blood sample just before the infusion or at least 2 weeks after the infusion of Aflibercept.
• This ELISA kit is used for in vitro qualitative determination of antibody against Aflibercept in serum and plasma
Kit Components • Micro ELISA Plate
• Positive Control
• Negative Control
• Assay Buffer
• Peroxidase Conjugate
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Aflibercept is a recombinant protein composed of the binding domains of two human vascular endothelial growth factor (VEGF) receptors fused with the Fc region of human immunoglobulin gamma 1 (IgG1). Aflibercept, as an ophthalmic agent, is used in the treatment of macular edema following Central Retinal Vein Occlusion (CRVO) and neovascular Age-Related Macular Degeneration (AMD). Compared to other anti-VEGF drugs like bevacizumab and ranibizumab, aflibercept has a higher binding affinity to VEGF-A. BioVision’s BioSim™ anti- Aflibercept ELISA kit is designed to detect antibody against Aflibercept with high specificity and sensitivity in serum and plasma samples. anti-Aflibercept ELISA is based on the sandwich ELISA principle. Controls and samples are incubated in the microtiter plate coated with the drug aflibercept. After incubation, the wells are washed. Then, HRP conjugated probe is added and binds to aflibercept antibodies captured by the drug aflibercept on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of aflibercept antibodies in the sample or controls. The results can be evaluated with using cut-off value.

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