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BioSim™ Aflibercept (Eylea®)(Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of antibody against Aflibercept in human serum or plasma.
Catalog #: E4854

Product Details

Cat # +Size E4854-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of Aflibercept in human serum and plasma
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of Aflibercept
• Recovery rate: <100±30% with normal human serum samples
• Cross Reactivity: Except for Aflibercept, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.
• Detection Range: 30 - 1000 ng/ml
• Sensitivity: 30 ng/ml
• Assay Precision: Intra-Assay: CV < 30%; Inter-Assay: CV < 30% (CV (%) = SD/mean X 100)
Kit Components • Micro ELISA Plate
• Aflibercept Standards (S1 – S7)
• Assay Buffer
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Aflibercept is a recombinant protein composed of the binding domains of two human vascular endothelial growth factor (VEGF) receptors fused with the Fc region of human immunoglobulin gamma 1 (IgG1). Aflibercept, as an ophthalmic agent, is used in the treatment of macular edema following Central Retinal Vein Occlusion (CRVO) and neovascular Age-Related Macular Degeneration (AMD). Compared to other anti-VEGF drugs like bevacizumab and ranibizumab, aflibercept has a higher binding affinity to VEGF-A. BioSim™ Aflibercept ELISA kit has been developed for specific quantification of Aflibercept concentration in human serum or plasma with high sensitivity and reproducibility. Aflibercept ELISA kit is based on the sandwich ELISA principle. Standards and samples (serum or plasma) are added in the microtiter plate coated with the reactant for aflibercept. After incubation, the wells are washed. The HRP conjugated probe is added and binds to aflibercept captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of aflibercept in the sample or standard. Results of samples can be determined directly using the standard curve.

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