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Bioluminescence Cytotoxicity Assay Kit

based on 2 citations in multiple journalsBioluminescence Cytotoxicity Assay Kit24.1 4
Cytotoxicity assay based on adenylate kinase (AK), HTS
Catalog #: K312

In stock

$155.00

Product Details

Cat # +Size K312-500
Size 500 assays
Detection Method Luminescence
Species Reactivity Mammalian
Applications N/A
Features & Benefits • Simple one-step procedure; takes less than 30 minutes
• Fast and convenient
• The assay is highly sensitive and can be fully automatic for high throughput studies.
Kit Components • AK Detection Reagent (Lyophilized)
• AK Assay Buffer
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and rapidly release into culture medium upon damage of the plasma membrane. The Bioluminescence Cytotoxicity Assay Kit is based on the measurement of AK in a simple one-step procedure involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase that was released from the damaged cells. The second reaction utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is highly sensitive and can be fully automatic for high throughput.


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Can I lyse the cells with any detergent?
You can lyse cells completely with any cell lysis buffer containing detergent. For 100% lysis control, use the buffer, sonicate cells and homogenize if necessary so that you achieve complete lysis. Confirm under microscope. Then use this cell lysate to measure 100% lysis value. You can use this value to calculate percentage of 100% lysis control for all other samples. I would suggest using the same number of cells in your 100% lysis control as the samples.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Iskandar et al., Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts.  Toxicol. Sci., Sep 2015; 147: 207 - 221.
Elks CM et al (2009) Am J Physiol Renal Physiol.; 296: F298 - F305.
For more citations of this product click here
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