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Bilirubin ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Bilirubin concentrations in serum, plasma and other biological fluids.
Catalog #: E4794
$565.00

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity All
Applications in vitro quantitative determination of Bilirubin concentrations in serum, plasma and other biological fluids.
Features & Benefits • Sensitivity: 0.19 μg/mL
• Precision:Coefficient of variation is < 10%.
• Detection Range: 0.31-20 μg/mL
• Specificity: No Significant cross-reactivity or interference between Bilirubin and analogues was observed.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert’s syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts. BioVision’s Bilirubin ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Bilirubin. During the reaction, Bilirubin in the samples or standard competes with Bilirubin coated on the plate for binding to the anti- Bilirubin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Bilirubin. The concentration of Bilirubin in the samples can be calculated by comparing the OD of the samples to the standard curve.


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