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BI is a DFHBI derivative that displays high Broccoli™ binding affinity and photostability
Catalog #: B2798
SKU-Size Size Price Qty
B2798-500 500 μg
B2798-1 1 mg
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Product Details

Alternate Name (Z)-3-((1H-Benzo[d]imadazol-4-Yl)Methyl)- 5-(3,5-Difluoro-4-Hydroxybenzylidene)-2- Methyl-3,5-Dihydro-4H-Imidazol-4-One
Peptide Sequence N/A
Appearance Yellow solid
Molecular Formula C₁₉H₁₄F₂N₄O₂
Molecular Weight 368.3
Purity ≥98% by HPLC
Solubility >10 mg/ml DMSO
InChi N/A
InChi Key N/A-
PubChem CID N/A
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


BI is a derivative of DFHBI (Cat. No. B2797) that binds Broccoli™ with higher affinity and deliver improved cellular fluorescence. In cells, BI has been found to stabilize the Broccoli™ structure and promote its folding. Additionally, Broccoli™/BI complexes is significantly more photostable due to impaired light-induced photoisomerization, and rapid unbinding of photoisomerized cis-BI. BI is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Broccoli™ RNA tag without disrupting biological functions. Importantly, the optimized fluorescence properties of BI enable live single-molecule imaging of Broccoli™-tagged mRNA transcripts in mammalian cells.

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