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Benzo-Endonuclease

To digest all forms of DNA and RNA
Catalog #: 9253
SKU-Size Size Price Qty
9253-50 50 kU
$295.00
9253-100 100 kU
$395.00
More Sizes Get Quote

Product Details

Alternate Name Serratia marcescens Binuclease; unrestricted endonuclease
Source Genetically engineered Serratia marcescens endonuclease expressed in yeast cells
Molecular Weight ~30 kDa
Purity by SDS-PAGE ≥ 90%
Endotoxin Level Endotoxin free as determined by the LAL method
Biological Activity ≥ 20 kU/mg lyophilized powder
Reconstitution Instructions Reconstitute in 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl.
Specific Activity ≥ 1000 kU/mg protein
Handling Centrifuge the vial prior to opening.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Benzo-Endonuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Benzo-Endonuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester bond. Benzo-Endonuclease reduces nucleic acid load and viscosity in cell lysates during purification to facilitate the filtration/ultrafiltration of solutions, reduces processing time, improves the separation of pellets and supernatants in centrifugations and increases the efficiency of chromatographic purification, resulting in significantly higher yield and purity of cell-derived particles such as viruses, AAV vectors or inclusion bodies.


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