BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible

Only kit on the market that is compatible with higher conc. of reducing agents TCEP (20 mM), DTT (10 mM), β-ME (35 mM)
Catalog #: K819 | abID: ab207004

Product Details

abID ab207004
Cat # +Size K819-250
Size 250 assays
Detection Method Absorbance (562 nm)
Applications Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Features & Benefits • Simple & convenient.
• Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM).
• The assay is linear over a wide range of protein concentration (25-2000 μg/ml).
Kit Components • BCA Reagent A
• BCA Reagent B
• BSA Standard (2 mg/ml)
• Blocking Reagent (20 mg/tube)
• Blocking Reagent Buffer
Storage Conditions RT
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This kit is adapted from BioVision’s BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determining protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over the widest range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.