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Bacterial Counting Colorimetric Assay Kit

Catalog #: K511
SKU-Size Size Price Qty
K511-500 500 assays
K511-2500 2500 assays
More Sizes Get Quote

Product Details

Detection Method Absorbance (460 nm)
Species Reactivity Bacteria
Applications The assay is a simple method to quantify bacteria concentration and viability. Can detect bacterial growth as few as 10 bacteria per sample
Features & Benefits • Simple procedure; takes less than ~90 minutes
• Fast and convenient
Kit Components • Electrocoupling Solution (ECS)
• WST Reagent (lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


The Bacteria Counting Colorimetric Assay Kit is a simple and sensitive assay to quantify bacterial concentration and viability. The assay is based on the cleavage of a water soluble tetrazolium salt (WST) to formazan by mitochondrial dehydrogenases. The color produced is directly proportional to the number of viable bacteria and can be quantified using a microplate reader (Absorbance OD 460 nm). The assay is sensitive and can detect the growth of low density cultures starting with as few as 10 bacteria per well at the time of adding the reagent. The assay can also be used to count the number of living bacteria in a broth culture or to study bacterial viability in response to inhibitors, media types, antibiotics, growth and heterologous protein overexpression conditions.

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What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.