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Azithromycin ELISA Kit

A Competitive ELISA Kit to quantitatively measure Azithromycin in milk, honey, and tissue samples
Catalog #: E4945
$970.00

Product Details

Cat # +Size E4945-100
Size 96 assays
Kit Summary Azithromycin is a broad-spectrum antimicrobial drug that is used to treat respiratory, enteric, and genitourinary infections. It targets both gram-positive and gram-negative bacteria. The mode of action of this drug is that it binds to the 23S rRNA of the 50S ribosomal subunit of the bacteria. This prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. Antibiotics such as Azithromycin have been used in food animals and aquaculture either to treat illnesses or as growth promoters. However, the use of Azithromycin in food animals may result in the formation of resistant bacteria and may lead to antibiotic resistance in humans. BioVision’s Azithromycin ELISA kit is used to quantitatively measure Azithromycin in tissues, honey, and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Azithromycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Azithromycin in the samples.
Detection Method Absorbance (450 nm)
Species Reactivity Chicken, Duck
Applications Competitive ELISA kit for quantitative measurement of Azithromycin in milk, tissue, and honey samples
Features & Benefits • Cross reaction: Azithromycin 100%, Erythromycin 100%
• Sensitivity: 0.2ppb
• This ELISA kit is used for in vitro quantitative determination of Azithromycin
• Detection Limit: 10ppb for raw milk, 4ppb for honey (method 1) and 0.2ppb (method 2), 15ppb for tissue
Kit Components • Micro ELISA Plate Plate Sealer
• Standard (S0 – S5)
• Enzyme Conjugate (11X)
• Enzyme Conjugate dilution
• Substrate A
• Substrate B
• Stop Solution
• Wash Buffer (20X)
• Redissolving solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Azithromycin is a broad-spectrum antimicrobial drug that is used to treat respiratory, enteric, and genitourinary infections. It targets both gram-positive and gram-negative bacteria. The mode of action of this drug is that it binds to the 23S rRNA of the 50S ribosomal subunit of the bacteria. This prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. Antibiotics such as Azithromycin have been used in food animals and aquaculture either to treat illnesses or as growth promoters. However, the use of Azithromycin in food animals may result in the formation of resistant bacteria and may lead to antibiotic resistance in humans. BioVision’s Azithromycin ELISA kit is used to quantitatively measure Azithromycin in tissues, honey, and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Azithromycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Azithromycin in the samples.


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