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Avermectin ELISA Kit

A Competitive ELISA kit to quantitatively measure Avermectin in tissue and milk samples

WARNING: This product can expose you to chemicals including TMB & DMF, which are known to the State of California to cause cancer. For more information go to www.p65warnings.ca.gov/.
Catalog #: E4944
$815.00

Product Details

Cat # +Size E4944-100
Size 96 assays
Kit Summary Avermectin is a 16-membered macrocyclic lactone derivative that is produced as a fermented product by the soil-dwelling actinomycete Streptomyces avermitilis. Due to its anthelmintic and insecticidal properties, it is widely used to treat parasitic infections in humans and animals and also used as a pesticide in agriculture to prevent insect attacks on crops. The mode of action of the drug is that it binds to and opens the glutamine gated chloride channel on the invertebrate muscle cells and neurons. This causes an increased influx of chloride ions into the cells which lead to hyperpolarization of the membranes, and ultimately resulting in paralysis and death of the invertebrates. BioVision’s Avermectin ELISA kit is used to quantitatively measure Avermectin in tissue and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Avermectin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Avermectin in the samples.
Detection Method Absorbance (450 nm)
Species Reactivity Chicken, Pork, Duck
Applications Competitive ELISA kit to quantitatively measure Avermectin in tissue and milk samples
Features & Benefits • Cross reaction: Avermectin 100%, Ivermectin 25%, Eprinomectin 10%, Doramectin 6%
• Detection Limit: 4ppb for tissues; 5ppb for milk
• This ELISA kit is used for in vitro quantitative determination of Avermectin
• Sensitivity: 0.5ppb
Kit Components • Micro ELISA Plate Sample Treatment Solution
• Plate Sealer
• Standard (S0 – S5)
• Enzyme Conjugate (11X)
• Enzyme Conjugate dilution
• Substrate A
• Substrate B
• Stop Solution
• Wash Buffer (20X)
• Redissolving Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Avermectin is a 16-membered macrocyclic lactone derivative that is produced as a fermented product by the soil-dwelling actinomycete Streptomyces avermitilis. Due to its anthelmintic and insecticidal properties, it is widely used to treat parasitic infections in humans and animals and also used as a pesticide in agriculture to prevent insect attacks on crops. The mode of action of the drug is that it binds to and opens the glutamine gated chloride channel on the invertebrate muscle cells and neurons. This causes an increased influx of chloride ions into the cells which lead to hyperpolarization of the membranes, and ultimately resulting in paralysis and death of the invertebrates. BioVision’s Avermectin ELISA kit is used to quantitatively measure Avermectin in tissue and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Avermectin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Avermectin in the samples.


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