Product Details
| Cat # +Size | K449-50 |
|---|---|
| Size | 50 assays |
| Kit Summary | • Detection method- Flow cytometry and Fluorescence Microscopy • Species reactivity- Mammalian • Applications - Measurement of Autophagy processes - Screening compounds affecting Autophagy |
| Detection Method | Flow Cytometry (FL1/FL2) |
| Species Reactivity | Mammalian |
| Applications | • Measurement of Autophagy processes • Screening compounds affecting Autophagy |
| Features & Benefits | Measurement of cellular autophaghy processes |
| Kit Components | • Assay Buffer (10X) • Autophagy Stain (500X) • Cell Death Marker (500X) • Positive Control Reagent (10000X) |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Autophagy or autophagocytosis (“self-eating”) refers to a process of degradation of cytoplasmic components within lysosomes, a unique process mediated by autophagosomes. Autophagy consists of several sequential steps: sequestration, transport to lysosomes, degradation and eventual re-utilization of degradation products. Autophagosomes engulf a portion of cytoplasm, thus autophagy is generally thought to be a nonselective degradation system regulated by many different cellular signaling pathways. Autophagy functions as a stress response upregulated by nutrient and energy starvation, oxidative stress, or other harmful conditions (such as damage to organelles, protein aggregation and infection by pathogens). Dysfunction of autophagy is associated with many human cancers and neurodegenerative diseases. BioVision’s Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.
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I would like to know which autophagy protein the kit detects? What is the principle of detection?
The autophagy stain specifically labels autophagic vacuoles. It selectively accumulates in PaTu8902 subcellular fractions containing the lysosomal enzymes, acid phosphatase and cathepsin D, but not those containing the rough and smooth ER markers TRAM and cytochrome P450, respectively.
