ATPase Assay Kit (Colorimetric) (ab234055)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
ATPase Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Range
1 nmol/well - 5 nmol/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ATPase Assay Kit (Colorimetric) (ab234055) provides a quick and easy method for monitoring ATPase activity in cell and tissue lysates.
In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, in which phosphate reacts with malachite green, a strong, stable chromophore is generated (OD 650 nm).
The assay is simple, sensitive, high-throughput adaptable and can detect ATPase Activity less than 0.005 U/L.
ATPases catalyzes the decomposition of ATP into ADP and a free phosphate ion. This assay can be used in the study of the many classes of ATPases including Na+ /K+ -ATPase, H + /K+ -ATPase, Ca2+ -ATPase, etc.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K417 ATPase Activity Assay Kit (Colorimetric). K417-100 is the same size as the 100 test size of ab234055.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests 100 tests ATPase Assay Buffer 1 x 55ml 1 x 55ml ATPase Developer 1 x 3ml 1 x 3ml ATPase Positive Control 1 vial 1 vial ATP Standard II 2 vials 2 vials Phosphate Standard 1 x 0.5ml 1 x 0.5ml
Associated products
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Related Products
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab234055 has been referenced in 7 publications.
- Bhardwaj A et al. MMD-associated RNF213 SNPs encode dominant-negative alleles that globally impair ubiquitylation. Life Sci Alliance 5:N/A (2022). PubMed: 35135845
- Ogando DG et al. Inducible Slc4a11 Knockout Triggers Corneal Edema Through Perturbation of Corneal Endothelial Pump. Invest Ophthalmol Vis Sci 62:28 (2021). PubMed: 34190974
- Lakshmipathi J et al. Nephron-Specific Disruption of Polycystin-1 Induces Cyclooxygenase-2-Mediated Blood Pressure Reduction Independent of Cystogenesis. J Am Soc Nephrol 31:1243-1254 (2020). PubMed: 32300065
- Lee CH et al. Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus. Nat Commun 11:5772 (2020). PubMed: 33188191
- Zahoor A et al. ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production. Biotechnol Biofuels 13:185 (2020). PubMed: 33292464
- Boecker S et al. Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli. Biotechnol J 14:e1800438 (2019). PubMed: 30927494
- Eleftheriadis T et al. Energy handling in renal tubular epithelial cells of the hamster, a native hibernator, under warm anoxia or reoxygenation. Biomed Rep 9:503-510 (2018). PubMed: 30546878