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ATPase Activity Assay Kit (Colorimetric)

Catalog #: K417

Product Details

Cat # +Size K417-100
Size 100 assays
Kit Summary • Detection method- Colorimetric (OD 650 nm)
• Species reactivity- Mammalian
• Applications- The assay is designed to measure ATPase activity in biological samples with detection sensitivity ~5 mU/l
Detection Method Colorimetric (OD 650 nm)
Species Reactivity Mammalian
Applications Rapid assessment of ATPase activity in biological samples or recombinant ATPase preparations
Features & Benefits • Simple procedure; takes less than 1 hour
• Fast and convenient
Kit Components • ATPase Assay Buffer
• ATPase Substrate
• ATPase Developer
• Phosphate Standard (10 mM)
• ATPase Positive Control
Storage Conditions Multiple Temperatures
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


ATPase (Adenosine Triphosphatase: EC is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ -ATPase, H+/K+ -ATPase, Ca2+ -ATPase, etc. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+-ATPase deficiency increases anxiety-related behavior, while Ca2+ -ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases. BioVision’s ATPase Activity Assay kit provides a quick and easy method for monitoring ATPase activity in various samples. In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated (OD 650 nm). The assay is simple, sensitive, high -throughput adaptable and can detect ATPase Activity less than 0.005 U/L

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