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ATF4 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of ATF4 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5008
$875.00

Product Details

Cat # +Size E5008-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of ATF4 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Recovery range: 85 - 110% for normal human serum and plasma samples
● This Sandwich ELISA is highly sensitive and highly specific for the detection of ATF4 in human samples. There is no significant cross-reactivity or interference between ATF4 and analogues
● Sensitivity: 0.10 ng/ml
● Detection range: 0.16 – 10 ng/ml
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standards
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (100X)
● Antibody Dilution Buffer
● HRP Conjugate (100X)
● HRP Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Activating transcription factor 4 (ATF4) is 351 amino acid basic leucine zipper (bZIP) stress-responsive transcription factor that belongs to the cAMP response element-binding protein 2 (CREB 2) family of proteins. The protein contains a leucine zipper domain that is responsible for the formation of homo and heterodimers necessary for protein-protein interactions. ATF4 is stimulated under cellular stress conditions such as amino acid depletion by General Control Nonderepressible-2 (GCN2) and protein kinase R-like ER kinase (PERK). Both GCN2 and PERK phosphorylate eIF2α and inhibit global protein translation. However, phosphorylation of eIF2α stimulates ATF4 to switch on nonessential amino acid (NEAA) biosynthesis and promote transportation activity of amino acids to help cells cope with amino acid depletion. ATF4 plays a role in the differentiation of osteoblasts and may provide insights into the progression of skeletal diseases. ATF4 also plays a significant role in diseases such as diabetic retinopathy, Alzheimer’s, Parkinson’s, Huntington’s, and prion disease. BioVision’s ATF4 (Human) ELISA kit quantitatively measures ATF4 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ATF4 in the sample or standard.


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