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Asprosin (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure Asprosin in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4899
$695.00

Product Details

Cat # +Size E4899-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of IL-33 in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Detection range: 1.563 - 100 ng/ml
• Sensitivity: 0.938 ng/ml
• Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• This Sandwich ELISA is highly sensitive and highly specific for the detection of Asprosin in human samples. There is no significant cross-reactivity or interference between Asprosin and analogues
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (100 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Asprosin is a novel glucogenic adipokine that plays a critical role in glucose homeostasis. The protein is formed as a result of Furin/PACE (paired basic amino acid cleaving enzyme) serine endopeptidase mediated cleavage of the C-terminal domain of profibrillin. It is predominantly produced and secreted by white adipose tissue. Plasma Asprosin levels oscillate in a circadian manner; the levels are low during feeding and high during fasting. One of the main functions of Asprosin is to stimulate the production and release of glucose in the liver via the CREB pathway. Additionally, in the brain, the protein stimulates the activity of AgRP neurons and promotes increased appetite. Elevated Asprosin expression occurs in disorders such as polycystic ovary syndrome (POCS), type 2 diabetes, and obesity. Antibody-based treatment of Asprosin is known to reduce glucose and insulin levels in diabetic mice. Thus, Asprosin could serve as an efficient therapeutic target to treat metabolic disorders. BioVision’s Asprosin (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of Asprosin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of Asprosin in the sample or standard.


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