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Product Details
| Cat # +Size | K753-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Mammalian |
| Applications | This assay detects AST/ SGOT with a detection limit of 10 mU per well. |
| Features & Benefits | • Simple procedure • Fast, reliable and convenient • Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses. |
| Kit Components | • AST Assay Buffer • AST Enzyme Mix • Developer • AST Substrate • Glutamate Standard (0.1M) • AST Positive Control |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s AST Assay Kit, an amino group is transferred of from aspartate to ⍺-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of AST with a detection limit of 10 mU per well.
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Does this kit also measure glutaminase activity in the lysates? Could glutaminase also generate glutamate, which is detected by the probe?
The kit is specific to AST/SGOT and does not measure glutaminase activity from the lysate. If the end user is concerned about endogenous glutamate present in the samples, then include a control well without any substrate. Any OD reading from the control well can be subtracted from the sample OD readings.
Andre Apostolatos, Preclinical testing of 5-amino-1-((1R,2S,3S,4R)-2,3-dihydroxy-4-methylcyclopentyl)-1H-imidazole-4-carboxamide: a potent protein kinase C-ι inhibitor as a potential prostate carcinoma therapeutic. Anticancer drugs, Jan 2019; 30204596.
Haibao Zhu, Spatial control of in vivo CRISPR–Cas9 genome editing via nanomagnets. Nat Biomed Eng, Nov 2018; 30944431.
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Mahesh, Malleswarapu et al. (2016) Carrot Juice Administration Decreases Liver Stearoyl-CoA
Desaturase 1 and Improves Docosahexaenoic Acid Levels, but Not
Steatosis in High Fructose Diet-Fed Weanling Wistar Rats., Prev Nutr Food Sci. 2016 Sep;21(3):171-180.
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