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ARNTL (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of ARNTL in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4990
$795.00

Product Details

Cat # +Size E4990-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure ARNTL in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● Recovery range: 85 - 105% for normal human serum and plasma samples
● This Sandwich ELISA is highly sensitive and highly specific for the detection of ARNTL in human samples. There is no significant cross-reactivity or interference between ARNTL and analogues
● Detection range: 15.625 – 1000 pg/ml
● Sensitivity: 9.375 pg/ml
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL, BMAL1) is a basic helix-loop-helix transcription factor with 2 PAS domains. It is a critical component of the circadian clock. ARNTL contributes to the positive limb of the transcription-translation feedback loop. It heterodimerizes with CLOCK and induces the expression of downstream circadian clock genes Periods (Pers) and Cryptochromes (Crys) inside the nucleus. Upon synthesis in the cytoplasm, PER and CRY proteins bind to Casein Kinases (CKs), translocate back to the nucleus, and inhibit CLOCK-ARNTL transcriptional activity. ARNTL also regulates hypertension, diabetes, and obesity. Mutations and knockouts of ARNTL are associated with infertility, impaired glucose and lipid homeostasis, disturbed sleep patterns, reduced life span, and age-related pathologies. BioVision’s ARNTL (Human) ELISA kit quantitatively measures ARNTL in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ARNTL in the sample or standard.


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