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Arginase Activity Colorimetric Assay Kit

The most sensitive kit on the market
Catalog #: K755
$415.00

Product Details

Cat # +Size K755-100
Size 100 assays
Detection Method Absorbance (570 nm)
Applications Measurement of Arginase Activity in various tissues/cells
Analysis of urea cycle disorders
Features & Benefits • Simple, rapid & convenient
• The assay can detect Arginase Activity less than 0.2U/L in 96-well assay format.
Kit Components • Arginase Assay Buffer
• OxiRed™ Probe (in DMSO)
• Arginase Substrate (Lyophilized)
• Arginase Enzyme Mix (Lyophilized)
• Arginase Developer (Lyophilized)
• Arginase Converter Enzyme (Lyophilized)
• H2O2 Standard (0.88 M)
• Arginase Positive Control (Lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Arginase (EC 3.5.3.1) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body. Arginase has two isoforms: Arginase I and Arginase II. Arginase I is mainly present in the liver and plays an important part in the urea cycle whereas Arginase II is present in kidney and other tissues and regulates Arginine/Ornithine concentration. Arginase deficiency can lead to severe symptoms including neurological impairment, dementia and hyperammonemia. Analysis of Arginase activity is fundamental to the study of the urea metabolic pathway. BioVision’s Arginase Activity Assay kit is simple, sensitive and rapid. In this assay, Arginase reacts with arginine & undergoes a series of reactions to form an intermediate that reacts stoichiometrically with OxiRed™ Probe to generate the colored product (OD 570 nm). The kit can detect Arginase activity less than 0.2 U/L in 96-well assay format.


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Can catalase in the tissue lysates interfere with the assay by consuming the H2O2?
Endogenous enzymes in the samples, like catalase, can interfere with the assay. However, it will boil down to the relative abundance of catalase vs arginase and also the individual Km values. According to our data, we could detect Arginase activity in liver samples higher than the background.
I am having trouble getting the correct H2O2 standard curve for the Arginase Activity Colorimetric Assay Kit (K755-100). My standard curve is flat. Why?
Your standard values are very high and its reaching saturation. I just want to make sure that you are diluting your standard correctly. You will need to dilute the stock solution twice:1. Dilute 0.88M H2O2 provided to 10mM2. Dilute the 10mM solution to 1mM3. Now add 0,2,4,6,8 and 10ul to the wells for standard curve generation.
Could you tell us the information about the source of any component in K755-100 ?
For K755, the following components contains enzymes from the species mentioned. Enzyme mix: Aerococcus viridansDeveloper: Pig heart, bovine liverConverter: Canavalia ensiformisPositive control: bovine liver.
Can you test serum samples?
There is not much Arginase enzyme in serum under normal conditions. Arginase I is mainly present in the liver and plays an important part in the urea cycle whereas Arginase II is present in kidney and other tissues and regulates Arginine/Ornithine concentration. For testing serum samples, 1-40ul can be tested directly per well without any preparation.
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.