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Product Details
| Alternate Name | BPTI, Basic protease inhibitor, Pancreatic trypsin inhibitor |
|---|---|
| Gene Symbol | BPI |
| Accession # | P00974 |
| Source | E. coli |
| Appearance | Lyophilized |
| Physical Form Description | Lyophilized from 5 mg/ml solution in PBS |
| Molecular Weight | 9.7 kDa (36-93 aa + N-terminal Poly-his tag) |
| Purity by SDS-PAGE | ≥90% |
| Reconstitution Instructions | Reconstitute in water to a concentration of 1 mg/ml. The solution can then be diluted into PBS or other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA). Avoid repeated freezing and thawing cycles. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
BioVision’s Recombinant Bovine Aprotinin has been tested for its inhibitory activity against Trypsin and Plasmin using Trypsin Activity Colorimetric Assay Kit (K771-100) and Plasmin Inhibitor Screening Kit (Fluorometric) (K382-100). The inhibitory activity of Recombinant Bovine Aprotinin (IC50 = 10.7 nM) against Trypsin was comparable to that of native Bovine Aprotinin (IC50 = 9.6 nM). The IC50 of Recombinant Bovine Aprotinin against Plasmin was 15 nM. It has been found to inhibit SARS-CoV and SARS-CoV-2 in vitro.
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