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Apoptotic DNA Ladder Isolation Kit

based on 7 citations in multiple journalsApoptotic DNA Ladder Isolation Kit74.2 4
No genomic DNA contamination
Catalog #: K170
$385.00

Product Details

Cat # +Size K170-50
Size 50 assays
Detection Method Agarose gel electrophoresis (with Ethidium Bromide)
Species Reactivity Mammalian
Applications Extraction of apoptotic DNA ladders without interference of intact genomic DNA
Features & Benefits • Simple one-step procedure; takes only 2-4 hours
• Fast and convenient
• The kit can extract apoptotic DNA ladder from both tissues and cells with as little as 5% or less apoptotic cell population.
Kit Components • DNA Ladder Extraction Buffer
• Enzyme A Solution
• Enzyme B (Lyophilized)
• Ammonium Acetate Solution
• DNA Suspension Buffer
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apoptotic DNA Ladder Extraction Kit provides an easy and sensitive means for detecting DNA fragmentation in apoptotic cells. The new procedure selectively extract DNA ladders without interference of intact genomic DNA, which significantly increase the cell numbers that can be can be extracted and load on one agarose gel well, therefore increase the detection sensitivity.


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What is the difference between K120 and K170?
Both K120 and K170 have similar protocols. However, in K170, there is a centrifugation step at the end of the protocol at 16,000 g, which will eliminate the genomic DNA from the samples. Additionally, K170 requires more cells and therefore there will be more genomic DNA whereas in K120, the number of cells is less and therefore, interference from genomic DNA will be less.
Emel AKSOY, Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner. J Vet Med Sci, Dec 2018;  30541984.
Sumalatha, Kodandaram et al. (2017) ROS-mediated induction of apoptosis by benzoquinone embelin in human colon adenocarcinoma cells HT-29, J Complement Integr Med. 2017 Mar 17;14(2)
Hong, E. Ionizing Radiation Induces Cellular Senescence of Articular Chondrocytes via Negative Regulation of SIRT1 by p38 Kinase. J. Biol. Chem. 2010; 285: 1283-1295.
Nakajima A et al (2009) PNAS; 106: 12448 - 12452.
Papineni S et al. (2008) Mol. Pharmacol. 73: 553-565.
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