ApoBrdU-IHC DNA Fragmentation Assay Kit (ab287854)
Key features and details
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue sections
Overview
-
Product name
ApoBrdU-IHC DNA Fragmentation Assay Kit
See all DNA fragmentation kits -
Sample type
Cell culture extracts, Tissue sections -
Assay time
3h 00m -
Species reactivity
Reacts with: Mammals -
Product overview
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. ApoBrdU-IHC DNA Fragmentation Assay Kit (ab287854) is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H2O2/Urea tablets for color generation and methyl green solution for counter staining the cells.
Properties
-
Storage instructions
Please refer to protocols. -
Components 50 tests 200X Conjugate 1 x 35µl 5X Reaction buffer 1 x 1.75ml Anti-BrdUBiotin Antibody 1 x 275µl Blocking buffer 1 x 22ml BrdUTP 1 x 0.44ml DAB Tablets 6 vials H2O2/Urea Tablets 6 vials Methyl Green 1 x 6ml Positive and Negative Control Slides 2 units Proteinase K 1 x 0.11ml TdT Enzymes 1 x 41µl -
Research areas
-
Relevance
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab287854 has not yet been referenced specifically in any publications.