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Apaf-1 Antibody

based on 4 citations in multiple journalsApaf-1 Antibody44.1 4
Catalog #: 3018
$265.00

Product Details

Cat # +Size 3018-100
Size 100 µg
Antibody Target Apaf-1
Alternate Name APAF1, CED4, DKFZp781B1145, KIAA0413
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Synthetic peptide surrounding amino acid 1161 of human Apaf-1
Accession # O14727
Gene ID 20141188
Appearance Colorless liquid
Concentration 0.2 mg/ml
Formulation 100 µg (0.2 mg/ml) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 0.01% thimerosal.
Purification Affinity purified
Species Reactivity Human, mouse, rat, bovine, rabbit, monkey, horse
Application Western blot
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.


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Siu, M. P. and Alway, S. E. (2005) J. Physiol. 565: 309-323.
Takatani, T., et al. (2004) Am J Physiol Cell Physiol 10.1152/ajpcell.00042.2004.
Dirks, A. and Leeuwenburgh, C. (2002) Am. J. Physiol. 282:519-527.
Phaneuf, S. and Leeuwenburgh, C. (2002) Am. J. Physiol. 282:423-430.
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