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Anti-NMDAR Antibody (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of anti-NMDAR antibodies in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4987

Product Details

Cat # +Size E4987-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure Anti-NMDAR antibodies in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● Detection range: 1.563 – 100 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Sensitivity: 0.938 ng/ml
● This Sandwich ELISA is highly sensitive and highly specific for the detection of Anti-NMDAR antibodies in human samples. There is no significant cross-reactivity or interference between Anti-NMDAR antibodies and analogues
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (100 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antigen
● Antigen Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


N-methyl-D-aspartate (NMDA) receptor is a glutamate-gated cation channel present in the hippocampus and cerebral cortex. NMDAR plays a critical role in the development of the central nervous system, helps generate rhythms for breathing and locomotion, and regulates processes involved in learning, memory, and neuroplasticity. The receptor is activated when two different agonists bind to it: glutamate and glycine. Abnormal functions or altered functions of NMDAR lead to several disorders such as Parkinson’s, Alzheimer’s disease, epilepsy, schizophrenia, and Huntington’s disease. Autoantibodies generated against the NMDA receptor result in an autoimmune condition called Anti-NMDA receptor encephalitis. BioVision’s Anti-NMDAR antibody (Human) ELISA kit quantitatively measures Anti-NMDAR antibodies in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antigen are added to the wells pre-coated with capture antigen and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of Anti-NMDAR antibodies in the sample or standard.

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