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Anti-LIN28B Antibody

Polyclonal antibody to detect endogenous levels of LIN28B in human sample
Catalog #: A1685

In stock

$395.00

Product Details

Cat # +Size A1685-100
Size 100 µl
Antibody Target LIN28B
Alternate Name CSDD 2; CSDD2; FLJ16517; Lin 28 homolog B (C. elegans); Lin 28.2; Lin-28B; LIN28B; LN28B_HUMAN; Protein lin-28 homolog B
Host Rabbit
Antibody Type Polyclonal
Isotype IgG
Immunogen Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
Accession # Q6ZN17
Gene ID 389421
Appearance Colorless liquid
Concentration 1 mg/ml
Formulation In PBS, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Affinity purified
Species Reactivity Human
Application Western Blotting
Application & Usage WB 1:500-1:1000,ELISA (peptide) 1:20000-1:40000
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression. When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.


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