Anti-Lactoferrin antibody (ab15811)
Key features and details
- Rabbit polyclonal to Lactoferrin
- Suitable for: IP, ICC, IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Lactoferrin antibody
See all Lactoferrin primary antibodies -
Description
Rabbit polyclonal to Lactoferrin -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC, IHC-P, ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) (Human).
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General notes
Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified using immobilized antigenic proteins and pre adsorbed on an immobilized heterologus lactoferin matrix to remove the cross reacting antibodies. -
Primary antibody notes
Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ELISA | (1) |
Use at an assay dependent concentration. PubMed: 17244155
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WB | (1) |
1/500. Predicted molecular weight: 79 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. PubMed: 17244155 |
WB
1/500. Predicted molecular weight: 79 kDa. |
Target
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
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Alternative names
- GIG12 antibody
- Growth inhibiting protein 12 antibody
- HLF2 antibody
see all
Images
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Anti-Lactoferrin antibody (ab15811) at 1/500 dilution + Human Lactoferrin
Predicted band size: 79 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab15811 has been referenced in 20 publications.
- Kumagai Y et al. Antimicrobial peptide LL-37 ameliorates a murine sepsis model via the induction of microvesicle release from neutrophils. Innate Immun 26:565-579 (2020). PubMed: 32600088
- Mao Y et al. Processed human amniotic fluid retains its antibacterial activity. J Transl Med 17:68 (2019). PubMed: 30823930
- Liu L et al. Assessment of bioactivities of the human milk lactoferrin-osteopontin complex in vitro. J Nutr Biochem 69:10-18 (2019). PubMed: 31048205
- Pérez-Debén S et al. iTRAQ comparison of proteomic profiles of endometrial receptivity. J Proteomics 203:103381 (2019). PubMed: 31102758
- Kahn S et al. Exosomal MicroRNAs in Milk from Mothers Delivering Preterm Infants Survive in Vitro Digestion and Are Taken Up by Human Intestinal Cells. Mol Nutr Food Res 62:e1701050 (2018). PubMed: 29644801