Anti-Histone H4 (asymmetric di methyl R3) antibody (ab194683)
Key features and details
- Rabbit polyclonal to Histone H4 (asymmetric di methyl R3)
- Suitable for: IHC-P, WB, ICC/IF, Dot blot
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (asymmetric di methyl R3) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (asymmetric di methyl R3) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Dot blotmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: a wide range of other species -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (asymmetric di methyl R3). A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
Database link: P62805 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/2000. Predicted molecular weight: 11 kDa.
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ICC/IF |
1/50 - 1/200.
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 11 kDa. |
ICC/IF
1/50 - 1/200. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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Paraffin-embedded human rectal cancer tissue stained for Histone H4 (asymmetric di methyl R3) using ab194683 at 1/200 dilution in immunohistochemical analysis.
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Immunofluorescence staining of 293T cells stained for Histone H4 (asymmetric di methyl R3 with ab194683. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-Histone H4 (asymmetric di methyl R3) antibody (ab194683)
Lane 1 : HeLa cell lysate
Lane 2 : H4 protein
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 11 kDaBlocking buffer: 3% nonfat dry milk in TBST.
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Dot-blot analysis of all sorts of methylation peptides using ab194683.
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Paraffin-embedded rat brain tissue stained for Histone H4 (asymmetric di methyl R3) using ab194683 at 1/200 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab194683 has been referenced in 6 publications.
- Sung BY et al. Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1. J Clin Invest 132:N/A (2022). PubMed: 35040433
- Zhang Q et al. Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5. Basic Clin Pharmacol Toxicol 131:18-33 (2022). PubMed: 35470570
- Janisiak J et al. Protein Arginine Methyltransferase (PRMT) Inhibitors-AMI-1 and SAH Are Effective in Attenuating Rhabdomyosarcoma Growth and Proliferation in Cell Cultures. Int J Mol Sci 22:N/A (2021). PubMed: 34360791
- Zhang X et al. PRMT3 promotes tumorigenesis by methylating and stabilizing HIF1α in colorectal cancer. Cell Death Dis 12:1066 (2021). PubMed: 34753906
- Favia A et al. The Protein Arginine Methyltransferases 1 and 5 affect Myc properties in glioblastoma stem cells. Sci Rep 9:15925 (2019). PubMed: 31685892
- Jin Y et al. Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia. J Clin Invest 126:3961-3980 (2016). PubMed: 27643437