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Product Details
| Cat # +Size | A1676-100 |
|---|---|
| Size | 100 µl |
| Antibody Target | EHMT2 |
| Alternate Name | BAT8; C6orf30; G9A; KMT1C; NG36; Histone-lysine N-methyltransferase EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human EHMT2 |
| Accession # | Q96KQ7 |
| Gene ID | 10919 |
| Appearance | Colorless liquid |
| Concentration | 1 mg/ml |
| Formulation | In phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol |
| Purification | Affinity purified |
| Species Reactivity | Human, Mouse, Rat |
| Application | WB, IHC |
| Positive Control | WB: HeLa cell lystae, IHC: Mouse liver tissue |
| Application & Usage | WB 1:500-1:2000, IHC 1:50-1:200 |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins. Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro. However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing. G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex.
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