Anti-cGMP antibody (ab836)
Key features and details
- Sheep polyclonal to cGMP
- Suitable for: ELISA, RIA
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-cGMP antibody
See all cGMP primary antibodies -
Description
Sheep polyclonal to cGMP -
Host species
Sheep -
Specificity
This antibody reacts specifically with cyclic GMP. -
Tested applications
Suitable for: ELISA, RIAmore details -
Immunogen
Full length protein corresponding to cGMP. Succinylated cGMP conjugated to bovine serum albumin.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
RIA |
Use at an assay dependent concentration.
Able to detect and quantitate cGMP; 50% binding is achieved with a 1:6,400 dilution. |
|
AP |
Use at an assay dependent concentration.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. Able to detect and quantitate cGMP; 50% binding is achieved with a 1:6,400 dilution. |
AP
Use at an assay dependent concentration. |
Target
-
Relevance
Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG). -
Alternative names
- Cyclic GMP antibody
- Cyclic guanosine monophosphate antibody
- Guanosine 3 5 Cyclic Monophosphate antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (2)
ab836 has been referenced in 2 publications.
- Wu W et al. Enhancing natriuretic peptide signaling in adipose tissue, but not in muscle, protects against diet-induced obesity and insulin resistance. Sci Signal 10:N/A (2017). PubMed: 28743802
- Yu L et al. A novel bioassay for the activity determination of therapeutic human brain natriuretic peptide (BNP). PLoS One 7:e49934 (2012). ELISA . PubMed: 23185490