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Anti-Cdc20 Antibody (CDC20/1102)

Monoclonal Antibody to detect Cdc20 in human samples
Catalog #: A1572

In stock

$395.00

Product Details

Cat # +Size A1572-100
Size 100 µg
Antibody Target Cdc20
Alternate Name CDC20, CDC20A, p55CDC, P55CDC-LSB, Cell division cycle protein 20 homolog
Host Mouse
Antibody Type Monoclonal
Isotype Mouse / IgG1, kappa
Immunogen Recombinant human Cdc20 protein
Accession # Q12834
Gene ID 991
Appearance Colorless liquid
Formulation Supplied in 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Protein A/G purification
Species Reactivity Human
Application FC, IF, IHC
Positive Control IHC: human Tonsil, human Gastric Carcinoma
Application & Usage FC: 0.5-1 µg/1X106 cells in 0.1ml, IF: 0.5-1 µg/ml, IHC: 2-4 µg/ml for 30 minutes at RT
Handling The antibody solution should be gently mixed before use
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.


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