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Anti-CD3e Antibody (C3e/1308)

Monoclonal Antibody to detect CD3e in human samples
Catalog #: A1523

Product Details

Cat # +Size A1523-100
Size 100 µg
Antibody Target CD3e
Alternate Name CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex
Host Mouse
Antibody Type Monoclonal
Isotype Mouse / IgG2b, kappa
Immunogen Recombinant human CD3e fragment (aa 23-119)
Accession # P07766
Gene ID 916
Appearance Colorless liquid
Formulation In 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Purified from rabbit anti-serum by Protein A
Species Reactivity Human
Application FC, IF, WB, IHC
Positive Control IHC: human Tonsil, WB: CD3e and Jurkat cell
Application & Usage FC: 0.5-1 µg/1X106 cells, IF: 1-2 µg/ml, IHC: 1-2 µg/ml for 30 minutes at RT, WB: 1-2 µg/ml for 60 minutes at RT
Handling The antibody solution should be gently mixed before use
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Recognizes the γ-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

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