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Anti-CD31 / PECAM-1 Antibody (C31.7)

Monoclonal Antibody to detect CD31 / PECAM-1 in human, Cynomolgus Monkey and Rabbit samples. Weakly reacts with Rat.
Catalog #: A1496
$395.00

Product Details

Cat # +Size A1496-100
Size 100 µg
Antibody Target CD31 / PECAM-1
Alternate Name EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'
Host Mouse
Antibody Type Monoclonal
Isotype Mouse / IgG1, kappa
Immunogen Human recombinant CD31 protein
Accession # P16284
Gene ID 5175
Appearance Colorless liquid
Formulation In 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Protein A/G purification
Species Reactivity Human, Cynomolgus Monkey and Rabbit. Weakly reacts with Rat.
Application FC, IF, IHC, WB
Positive Control IHC: human Angiosarcoma, FC: human CD31 on Jurkat Cells, WB: CD31 in THP-1 Lysate
Application & Usage FC: 0.5-1ug/1X106 cells in 0.1ml, IF: 0.5-1ug/ml, WB: 0.5-1.0ug/ml, IHC: 1-2 ug/ml for 30 minutes at RT
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence


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