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Anti-CD22 / BL-CAM Antibody (BLCAM/1795)

Monoclonal Antibody to detect CD22 / BL-CAM in human samples
Catalog #: A1482

In stock


Product Details

Cat # +Size A1482-100
Size 100 µg
Antibody Target CD22 / BL-CAM
Alternate Name B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14
Host Mouse
Antibody Type Monoclonal
Isotype Mouse / IgG1, kappa
Immunogen Recombinant human BL-CAM/CD22 protein (aa52-178) (exact sequence is proprietary)
Accession # P20273
Gene ID 933
Appearance Colorless liquid
Formulation In 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Protein A/G purification
Species Reactivity Human
Application FC, IF, IHC, WB, ELISA
Positive Control IHC: human Tonsil, WB: Analysis of Raji and Ramos Cell Lysates
Application & Usage ELISA: Use Ab at 2-4ug/ml for coating, FC: 0.5-1ug/1X106 cells, IF: 0.5-1ug/ml, IHC: 1-2ug/ml for 30 minutes at RT, WB: 0.5-1ug/ml
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

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