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Anti-Caldesmon Antibody (SPM168)

Monoclonal antibody to detect Caldesmon in human samples.
Catalog #: A1452

In stock

$325.00

Product Details

Cat # +Size A1452-100
Size 100 µg
Antibody Target Caldesmon
Alternate Name CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1, kappa
Immunogen Crude human uterus extract
Accession # Q05682
Gene ID 800
Appearance Colorless liquid
Formulation In 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Protein A/G purification
Species Reactivity Human
Application FC, IF, IHC
Positive Control IHC: human Uterus
Application & Usage FC: 0.5-1 ug/1X106 cells, IF: 1-2 ug/ml, IHC: 0.25-0.5 ug/ml
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.


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