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Anti-BrdU Antibody (3E2D3)

BrdU monoclonal antibody for studying cell proliferation, cytokinetics etc.
Catalog #: A1284
$320.00

Product Details

Cat # +Size A1284-100
Size 100 µg
Antibody Target BrdU
Alternate Name BrdU, BUdR, BrdUrd
Host Mouse
Antibody Type Monoclonal
Isotype MouseIgG1 ĸ
Immunogen BrdU conjugated to KLH
Appearance Colorless liquid
Formulation In PBS, pH 7.4 containing 0.02% sodium azide
Purification Affinity purified
Application ELISA, FC, ICC/IF
Positive Control FC: Hela cells, ICC/IF: Hela cells
Application & Usage ELISA: 0.015- 0.05 µg/ml, Flow cytometry: 1-3 µg for 1 x 106 cells, ICC/IF: 1-3 µg/ml
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange. BrdU monoclonal antibody, is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.


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