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Anti-Biotin Antibody (Hyb-8)

Monoclonal antibody to detect Biotin in samples
Catalog #: A1441

In stock

$325.00

Product Details

Cat # +Size A1441-100
Size 100 µg
Antibody Target Biotin
Alternate Name Vitamin B7, Vitamin H
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1
Immunogen Biotinylated sheep immunoglobulin
Appearance Colorless liquid
Formulation In 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Protein A/G purification
Species Reactivity All species
Application FC, IF, IHC, WB, Confocal Microscopy, In situ Nucleic Acid Hybridization
Positive Control IHC: human tonsil
Application & Usage Confocal Microscopy, FC: 0.5-1 ug/1X106 cells, IF: 1-2 ug/ml, WB, IHC: 0.5-1 ug/ml for 30 minutes at RT, In situ Nucleic Acid Hybridization
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.


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