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Anti-Arginase 1 Antibody (ARG1/1125)

Monoclonal antibody to detect Arginase 1 in human samples
Catalog #: A1430

In stock

$325.00

Product Details

Cat # +Size A1430-100
Size 100 µg
Antibody Target Arginase 1
Alternate Name Arginase 1, ARG1, liver-type arginase, type I arginase
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG3, kappa
Immunogen Recombinant fragment (87 Amino acid residues around aa 1-150) of human ARG1 protein
Accession # P05089
Gene ID 383
Appearance Colorless liquid
Formulation In 10 mM PBS with 0.05% BSA & 0.05% azide
Purification Protein A/G purification
Species Reactivity Human
Application FC, IF, IHC, WB
Positive Control IHC: human hepatocellular carcinoma, WB: Recombinant ARG1 protein fragment, human liver lysate
Application & Usage FC: 0.5-1 ug/1X106 cells, IF: 1-2 ug/ml, WB: 0.5-1ug/ml, IHC (Formalin-fixed): 1-2 ug/ml
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.


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