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Anti-Akt1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody to detect Akt1 in human samples.
Catalog #: A1117
$285.00

Product Details

Cat # +Size A1117-50
Size 50 µl
Antibody Target AKT1
Alternate Name PKB, RAC, Protein kinase B, Protein kinase B alpha, Short PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Host Rabbit
Antibody Type Monoclonal
Isotype Rabbit IgG
Immunogen A synthetic peptide corresponding to the C-terminus of human Akt1
Accession # P31749
Gene ID 207
Appearance Colorless liquid
Formulation In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Purification Affinity purified using protein A
Species Reactivity Human
Application IHC, WB
Positive Control WB: 293 cell lysates, IHC: human breast cancer tissue sections
Application & Usage IHC: 1:500 -1:1000 dilution; WB: 1:1000 - 1:2000 dilution
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.


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